Bladder cancer (BC) is the most prevalent malignant tumor in the urinary tract, classified mainly into muscle-invasive BC (MIBC) and non-MIBC (NMIBC). Recent studies highlight the important role of changes in transcriptome activity in carcinogenesis, aiding in the identification of additional differentially regulated candidate genes, improving our understanding of the molecular basis of gene regulation in BC.

Pietro Pepe and the team aimed to evaluate the transcriptome of patients with MIBC compared with normal subjects.

They performed an inclusive analysis utilizing mRNA sequencing on the Illumina NovaSeq 6000 Dx system in a case series of 11 subjects with MIBC and 19 age- and sex-matched healthy controls. Functional analysis employed the pathfindR package to comprehensively identify enriched pathways within active subnetworks in omics data.

Our results demonstrated differentiated pathways, including spliceosome activity, oxidative phosphorylation, and chemical carcinogenesis due to reactive oxygen species, in patients with MIBC compared with controls.

The study concluded significant potential for defining cancer predisposition factors and exploring therapeutic options through the identification of novel molecular pathways in patients with MIBC.

No funding information was provided.

Bladder

Source: https://pubmed.ncbi.nlm.nih.gov/38936905/

Pepe P, Salemi M, Marchese G, et al. (2024). “Transcriptome Analysis in Patients With Muscle-invasive Bladder Cancer.” In Vivo. 2024 Jul-Aug;38(4):1660-1664. doi: 10.21873/invivo.13615. PMID: 38936905.