KEY TAKEAWAYS
- The study aimed to investigate the cellular impact of siRNA-mediated BATF3 downregulation on CML.
- Researchers noticed that the suppression of BATF3 emerges as a promising targeted therapy strategy for CML, highlighting its potential for improved treatment outcomes.
Despite advancements in chronic myeloid leukemia (CML) treatment, resistance renders it incurable. Investigating CML’s molecular mechanisms offers hope for highly efficient targeted therapies. The leucine zipper transcription factor BATF3, a key cellular regulator, influences tumor development in various cancers.
Reza Dabbaghipour and his team aimed to assess the impact of siRNA-mediated BATF3 downregulation on CML cells, focusing on cell proliferation, apoptosis induction, and cell cycle distribution.
They performed an inclusive analysis by employing an electroporation device for BATF3 siRNA transfection into K562 CML cells. Cellular viability and apoptosis were measured using MTT assay and Annexin V/PI staining. Cell cycle distribution of K562 cells was assessed through a cell cycle assay and flow cytometry instrument. Furthermore, for additional validation, mRNA expression of correlated genes was relatively evaluated using quantitative real-time polymerase chain reaction (qRT-PCR).
siRNA-mediated BATF3 inactivation significantly promoted cell apoptosis in K562 CML cells. The targeted therapy resulted in a notable increase in the expression of the Caspase-3 gene. The Bax/Bcl-2 ratio was also elevated, indicating a shift towards pro-apoptotic signaling, silencing BATF3-induced cell cycle arrest in the sub-G1 phase compared to the control. Notably, c-Myc gene expression showed a significant decrement through the suppression of BATF3 in CML cells.
The study concluded that the suppression of BATF3 emerges as an effective targeted therapy strategy for CML, emphasizing its potential significance in advancing treatment options for improved patient outcomes.
No funding was provided for the study.
Source: https://pubmed.ncbi.nlm.nih.gov/38217769/
Dabbaghipour R, Khaze Shahgoli V, Safaei S, et. al(2024) ‘’siRNA-mediated downregulation of BATF3 diminished proliferation and induced apoptosis through downregulating c-Myc expression in chronic myelogenous leukemia cells.’’ Mol Biol Rep. 2024 Jan 13;51(1):100. doi: 10.1007/s11033-023-09059-z. PMID: 38217769.
